A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum | Zendy

Cristina Pacios Bras | Zendy; Meritxell Alberich-Jordà | Zendy; André H. M. Wijfjes | Zendy; Marga Harteveld | Zendy; Nico Stuurman | Zendy; Jane ThomasOates | Zendy; Herman P. Spaink | Zendy

Open Access

A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum

Author(s) -

Cristina Pacios Bras,

Meritxell Alberich-Jordà,

André H. M. Wijfjes,

Marga Harteveld,

Nico Stuurman,

Jane ThomasOates,

Herman P. Spaink

Publication year - 2000

Publication title -

molecular plant-microbe interactions

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.565

H-Index - 153

eISSN - 1943-7706

pISSN - 0894-0282

DOI - 10.1094/mpmi.2000.13.4.475

Subject(s) - rhizobium leguminosarum , biology , heterologous expression , transferase , lotus japonicus , rhizobium , heterologous , biochemistry , rhizobiaceae , symbiosis , gene , recombinant dna , bacteria , enzyme , mutant , genetics

Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizo-bium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-α-(2-aminoethoxyvinyl) glycine (AVG).

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research