Open AccessAn HR-Induced Tobacco Peroxidase Gene Is Responsive to Spermine, but Not to Salicylate, Methyl Jasmonate, and Ethephon
Author(s) -
Susumu Hiraga,
Hiroyuki Ito,
Hiromoto Yamakawa,
Norihiro Ohtsubo,
Shigemi Seo,
Ichiro Mitsuhara,
Hirokazu Matsui,
Mamoru Honma,
Yuko Ohashi
Publication year - 2000
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi.2000.13.2.210
Subject(s) - methyl jasmonate , ethephon , tobacco mosaic virus , salicylic acid , gene , peroxidase , nicotiana tabacum , gene expression , methyl salicylate , biology , chemistry , inducer , microbiology and biotechnology , biochemistry , virus , genetics , enzyme , botany , ethylene , catalysis
In Tobacco mosaic virus (TMV)-infected tobacco plants carrying the N resistance gene, a hypersensitive reaction or response (HR) occurs to enclose the virus in the infected tissue. Although a contribution of peroxidases to the resistance has been proposed, no evidence has been presented that tobacco peroxidase genes respond to HR. Here, we describe the HR-induced expression of a tobacco peroxidase gene (tpoxC1) whose induction kinetics were slightly different from those of acidic and basic tobacco pathogenesis-related (PR) protein genes. Interestingly, tpoxC1 was insensitive to the inducers of PR genes such as salicylic acid, methyl jasmonate, and ethephon. Spermine activated tpoxC1 gene expression at a low level and both acidic and basic PR gene expression at a considerably higher level. These results indicate that the induced expression of tpoxC1 is regulated differently from that of classical tobacco PR genes in the N gene-mediated self-defense system in tobacco plants.