Open AccessAvirulence in the Wheat Septoria tritici Leaf Blotch Fungus Mycosphaerella graminicola Is Controlled by a Single Locus
Author(s) -
Gert H. J. Kema,
E.C.P. Verstappen,
C. Waalwijk
Publication year - 2000
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi.2000.13.12.1375
Subject(s) - septoria , mycosphaerella graminicola , biology , graminicola , cultivar , locus (genetics) , mycosphaerella , heterothallic , virulence , fungus , inoculation , botany , horticulture , genetics , mating type , pathogen , gene
Segregation of avirulence in Mycosphaerella graminicola, a heterothallic ascomycete that causes wheat septoria tritici leaf blotch, was studied in F1, BC1, and F2 populations by inoculation assays on five wheat cultivars in the seedling stage and by amplified fragment length polymorphism and random amplified polymorphic DNA analyses. F1 was generated by crossing isolates IPO323 (avirulent) and IPO94269 (virulent). All F1, BC1, and F2 progeny isolates were virulent on the susceptible check cultivar Taichung 29 and were avirulent on the resistant check cultivar Kavkav-K4500. Avirulence segregation was observed in F1 and in several BC1 and F2 generations on the differential cultivars Shafir, Kavkaz, and Veranopolis at a 1:1 ratio. Avirulence for the three differential cultivars always cosegregated. We conclude that avirulence in isolate IPO323 is controlled by a single, seemingly complex locus.