Open AccessStructural and Expression Analysis of Uricase mRNA from Lotus japonicus
Author(s) -
Kenichi Takane,
Shigeyuki Tajima,
Hiroshi Kouchi
Publication year - 2000
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi.2000.13.10.1156
Subject(s) - lotus japonicus , biology , parenchyma , lotus , root nodule , nodule (geology) , phylogenetic tree , complementary dna , legume , botany , microbiology and biotechnology , gene , biochemistry , genetics , nitrogen fixation , paleontology , mutant , bacteria
Uricase (nodulin-35) cDNA, LjUr, was isolated from nodules of a model legume, Lotus japonicus. LjUr expression was most abundant in nodules, although it was detected in nonsymbiotic tissues as well, particularly in roots. Expression in nodules was detected in uninfected cells, nodule parenchyma, and, more intensely, in vascular bundles. Phylogenetic analysis of uricase sequences from various legumes indicated that uricases of amide- and ureide-transporting legumes form two distinct clades. LjUr is in the cluster of amide-transport legumes even though L. japonicus bears determinate nodules.