Open AccessEndophytic Expression of nif Genes of Azoarcus sp. Strain BH72 in Rice Roots
Author(s) -
Tanja Egener,
Thomas Hurek,
Barbara ReinholdHurek
Publication year - 1999
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi.1999.12.9.813
Subject(s) - endophyte , nitrogenase , oryza sativa , diazotroph , nitrogen fixation , biology , gene , botany , strain (injury) , microbiology and biotechnology , bacteria , genetics , anatomy
Transcriptional fusions of gusA and gfp to the nifH gene as well as immunogold labeling with antibodies against the iron protein of nitrogenase revealed high nitrogenase gene expression levels of the endophyte Azoarcus sp. strain BH72 inside infected rice roots (Oryza sativa) in the laboratory. Thus, environmental conditions inside rice roots are permissive for endophytic nitrogen fixation in bacterial microcolonies in the aerenchyma.