Magnaporthe grisea Pathogenicity Genes Obtained Through Insertional Mutagenesis | Zendy

James A. Sweigard | Zendy; Anne Carroll | Zendy; Leonard Farrall | Zendy; Forrest G. Chumley | Zendy; Barbara Valent | Zendy

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Magnaporthe grisea Pathogenicity Genes Obtained Through Insertional Mutagenesis

Author(s) -

James A. Sweigard,

Anne Carroll,

Leonard Farrall,

Forrest G. Chumley,

Barbara Valent

Publication year - 1998

Publication title -

molecular plant-microbe interactions

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.565

H-Index - 153

eISSN - 1943-7706

pISSN - 0894-0282

DOI - 10.1094/mpmi.1998.11.5.404

Subject(s) - magnaporthe grisea , insertional mutagenesis , biology , hygromycin b , mutant , gene , genetics , mutagenesis , mutation , pathogenicity , cloning (programming) , microbiology and biotechnology , oryza sativa , computer science , programming language

We have initiated a mutational analysis of pathogenicity in the rice blast fungus, Magnaporthe grisea, in which hygromycin-resistant transformants, most generated by restriction enzyme-mediated integration (REMI), were screened for the ability to infect plants. A rapid primary infection assay facilitated screening of 5,538 transformants. Twenty-seven mutants were obtained that showed a reproducible pathogenicity defect, and 18 of these contained mutations that cosegregated with the hygromycin resistance marker. Analysis of eight mutants has resulted in the cloning of seven PTH genes that play a role in pathogenicity on barley, weeping lovegrass, and rice. Two independent mutants identified the same gene, PTH2, suggesting nonrandom insertion of the transforming DNA. These first 7 cloned PTH genes are described.

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