Accumulation of Transcripts Encodinga Lipid Transfer-Like Protein During Deformation ofNodulation-CompetentVigna unguiculataRoot Hairs | Zendy

Andréa Krause | Zendy

Open Access

Accumulation of Transcripts Encodinga Lipid Transfer-Like Protein During Deformation ofNodulation-CompetentVigna unguiculataRoot Hairs

Author(s) -

Andréa Krause

Publication year - 1994

Publication title -

molecular plant-microbe interactions

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.565

H-Index - 153

eISSN - 1943-7706

pISSN - 0894-0282

DOI - 10.1094/mpmi-7-0411

Subject(s) - vigna , biology , complementary dna , root hair , rhizobium , hordeum vulgare , isoelectric point , root nodule , signal peptide , plant lipid transfer proteins , botany , peptide sequence , microbiology and biotechnology , bacteria , biochemistry , symbiosis , gene , genetics , poaceae , enzyme

A cDNA library was constructed from RNA of Vigna unguiculata root hairs harvested 1 day and 4 days after inoculation with Rhizobium sp. NGR234. A heterologous probe was used to identify a cDNA clone, the predicted 99-amino-acid sequence of which shares homology with a nonspecific lipid transfer protein (LTP) of Hordeum vulgare. Other characteristics, including an estimated molecular weight of 10.4 kD, an isoelectric point of 8.6, and a signal peptide with a hydrophobic region at the amino-terminal end, are shared by most LTPs. A transcript of 630 nt was found in all tissues tested, except nodules. Levels of mRNA increased in root hairs 24 hr after treatment with Rhizobium sp. NGR234, with different hormones, or with Nod factors. Amounts of transcripts were dependent on the concentration of Nod factors. Accumulation of transcripts during nodule development correlated with root hair deformation, the first visible step in the Rhizobium-legume symbiosis.

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