Open AccessAgrobacterium rhizogenes Transformation of the Phaseolus spp.: A Tool for Functional Genomics
Author(s) -
Georgina Estrada-Navarrete,
Xóchitl AlvaradoAffantranger,
Juan-Elías Olivares,
Claudia Díaz-Camino,
Olivia Santana,
Enrique G. Murillo,
Gabriel Guillén,
Nayeli Sánchez-Guevara,
Jorge Acosta,
Carmen Quinto,
Dongxue Li,
Peter M. Gresshoff,
Federico Sánchez
Publication year - 2006
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi-19-1385
Subject(s) - phaseolus , agrobacterium , transformation (genetics) , biology , functional genomics , botany , inoculation , rhizobiaceae , cultivar , rhizobium , horticulture , genomics , symbiosis , genetics , gene , genome , bacteria
A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, landraces, and cultivars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. Induced hairy roots are robust and grow quickly. The transformation frequency is between 75 and 90% based on the 35-S promoter-driven green fluorescent protein and β-glu-curonidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal determinate nodules that fix nitrogen as efficiently as inoculated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiology, root metabolism, and root–microbe interactions.