Open AccessGmCOI1, a Soybean F-Box Protein Gene, Shows Ability to Mediate Jasmonate-Regulated Plant Defense and Fertility in Arabidopsis
Author(s) -
Zhilong Wang,
Liangying Dai,
Zide Jiang,
Peng Wen,
Lian-Hui Zhang,
Guoliang Wang,
Daoxin Xie
Publication year - 2005
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi-18-1285
Subject(s) - arabidopsis , jasmonate , biology , mutant , skp1 , gene , f box protein , gene family , genetics , methyl jasmonate , botany , microbiology and biotechnology , gene expression , ubiquitin , ubiquitin ligase
The F-box protein gene COI1 from Arabidopsis plays a fundamental role in response to jasmonates, which regulate plant root growth, pollen fertility, wounding and healing, and defense against pathogens and insects. Null mutations in COI1 were previously found to abolish all the jasmonate responses, and the Arabidopsis coi1-1 mutant is male sterile and susceptible to pathogen infection. In this study, we isolated an F-box protein gene from soybean, which shares significant homology with the Arabidopsis COI1 and similarly contains an F-box motif and leucine rich repeats (LRR), here designated GmCOI1 (Glycine max L. (Merr.) COI1). To test whether the sequence homology and structural similarity are indicative of functional conservation, we expressed GmCOI1 in the Arabidopsis coi1-1 mutant. The transgenic coi1-1 plants with expression of the GmCOI1 gene were found to exhibit normal jasmonate responses, including jasmonate-regulated plant defense and fertility. In addition, the chimerical proteins with swapped domain of the F-box motif or LRR between GmCOI1 and COI1 were shown to functionally complement the coi1-1 mutation. Furthermore, GmCOI1 was found to assemble into the Skp1-Cullin-F-box (SCF) complexes, similar to the formation of the Arabidopsis SCF COI1 . These data demonstrate the soybean F-box protein gene GmCOI1 is able to mediate jasmonate-regulated plant defense and fertility in Arabidopsis, which implies a generic jasmonate pathway with conserved signal components in different plant species.