Open AccessFunctional Analysis of Pathogenicity Proteins of the Potato Cyst Nematode Globodera rostochiensis Using RNAi
Author(s) -
Qing Chen,
Sajid Ur Rehman,
G. Smant,
John T. Jones
Publication year - 2005
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi-18-0621
Subject(s) - globodera rostochiensis , rna interference , potato cyst nematode , biology , nematode , globodera pallida , pathogenicity , function (biology) , gene , host (biology) , rna silencing , microbiology and biotechnology , rna , genetics , ecology , solanaceae
RNA interference (RNAi) has been used widely as a tool for examining gene function and a method that allows its use with plant-parasitic nematodes recently has been described. Here, we use a modified method to analyze the function of secreted β-1,4, endoglucanases of the potato cyst nematode Globodera rostochiensis, the first in vivo functional analysis of a pathogenicity protein of a plant-parasitic nematode. Knockout of the β-1,4, endoglucanases reduced the ability of the nematodes to invade roots. We also use RNAi to show that gr-ams-1, a secreted protein of the main sense organs (the amphids), is essential for host location.