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Lastly, we describe the nearly complete sequence of the Wuhan Tick Virus-like (WTV-like), which is comprised of one negativesense single stranded RNA molecule, with 11,208 nucleotides. This virus was classified into Chuviridae family and encodes the polymerase, glycoprotein, nucleoprotein, and VP4. Interestingly, the tick pools from all the four sites of collection were positive for MGTV, LT2V-like, and WTV-like viruses, indicating that these viruses can be found in the tick population of a large area of the Brazilian territory, which is an important cattle producing region in the country and one of the leading regions in animal produce exportation. On the other hand, only the MGTV was simultaneously detected in 19.4 per cent (7/36) serum cattle, indicating viremia in these animals. In summary, we have identified three potentially novel tick-borne viruses with broad distributions in South of Brazil, which include potential novel pathogens for cattle.

A60 Molecular epidemiology of canine parvovirus type 2 (CPV-2) in Italy