A28 Phylogeographic analysis of HIV-2 ANRS CO5 cohort reveals new trends in HIV-2 epidemic patterns in West Africa

sion alters the mutational profile (frequency and spectra) of integrated proviruses. We will explore how mutation rates of HIV2 can be manipulated through the use of nucleoside analogs and RNRI drugs to explore what effects these compounds have on the HIV-2 mutation profile. Using single-cycle infectivity assays as well as long-term spreading experiments, we will be able to correlate mutagenesis with viral evolution and infectivity data to explore how sensitive these two viruses are to changes in viral mutation. This work will serve to understand how HIV-2 operates at a lower mutation frequency than HIV-1, elucidate the relationship between mutagenesis and infectivity for the two viruses, and provide insights into the contrasting phenotypes observed between the viruses.