Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014–15 influenza season | Zendy

Deena Blumenkrantz | Zendy; Thomas Mehoke | Zendy; Kathryn ShawSaliba | Zendy; Harrison Powell | Zendy; Nicholas Wohlgemuth | Zendy; Hsuan Liu | Zendy; Elizabeth Macias | Zendy; Jared Evans | Zendy; Mitra Lewis | Zendy; Rebecca Medina | Zendy; Justin Hardick | Zendy; Lauren Sauer | Zendy; Andrea Dugas | Zendy; Anna DuVal | Zendy; Andrew P. Lane | Zendy; Charlotte A. Gaydos | Zendy; Richard E. Rothman | Zendy; Peter Thielen | Zendy; Andrew Pekosz | Zendy

Open Access

Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014–15 influenza season

Author(s) -

Deena Blumenkrantz,

Thomas Mehoke,

Kathryn ShawSaliba,

Harrison Powell,

Nicholas Wohlgemuth,

Hsuan Liu,

Elizabeth Macias,

Jared Evans,

Mitra Lewis,

Rebecca Medina,

Justin Hardick,

Lauren Sauer,

Andrea Dugas,

Anna DuVal,

Andrew P. Lane,

Charlotte A. Gaydos,

Richard E. Rothman,

Peter Thielen,

Andrew Pekosz

Publication year - 2021

Publication title -

virus evolution

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.231

H-Index - 23

ISSN - 2057-1577

DOI - 10.1093/ve/veab047

Subject(s) - genotype , biology , clade , virology , virus , antigenic drift , influenza a virus , mutation , gene , disease , genetics , medicine , phylogenetics

The 2014–15 influenza season saw the emergence of an H3N2 antigenic drift variant that formed the 3C.2a HA clade. Whole viral genomes were sequenced from nasopharyngeal swabs of ninety-four patients with confirmed influenza A virus infection and primary human nasal epithelial cell cultures used to efficiently isolate H3N2 viruses. The isolates were classified by HA clade and the presence of a new set of co-selected mutations in NA (a glycosylation site, NAg+) and PB1-F2 (H75P). The NA and PB1-F2 mutations were present in a subset of clade 3C.2a viruses (NAg+F2P), which dominated during the subsequent influenza seasons. In human nasal epithelial cell cultures, a virus with the novel NAg+F2P genotype replicated less well compared with a virus with the parental genotype. Retrospective analyses of clinical data showed that NAg+F2P genotype viruses were associated with increased cough and shortness of breath in infected patients.

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