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The approach of this experiment was to apply the regression method for the estimation of endogenous intestinal losses of ether extract (EEE) when pigs are fed complete diets ad libitum and using dietary levels of fat typical of those employed in commercial situations. A total of 40 gilts (PIC 337 sires × C22 or C29) were allotted to individual pens and randomly assigned to diets (8 pigs per treatment) with 5 different levels of acid hydrolyzed ether extract (AEE). The dietary treatments consisted of a corn-soybean meal diet with no added fat (L1); a corn-soy diet with 6% each of corn distiller’s dried grains with solubles (DDGS), corn germ meal, and wheat middlings (L2); the L2 diet but with 12% each of corn DDGS, corn germ meal, and wheat middlings (L3); the L2 diet plus soybean oil to equalize the NE concentration of the L2 diet with L1 (L4); and the L3 diet plus soybean oil to equalize the NE concentration of the L3 diet with L1 (L5). Pigs received feed and water ad libitum for the growing period (initial BW = 38.5 ± 1.2 kg) and the finishing period (initial BW = 73.82 ± 2.9 kg). A quadratic broken-line model was employed to estimate the response of apparent total tract digestibility (ATTD) of AEE to dietary AEE level. The average true total tract digestibility (TTTD) of AEE and endogenous losses of AEE were estimated using regression analysis of dietary AEE intake (g/kg of DM) against apparent digested AEE (g/kg of DMI). The ATTD of AEE increased in curvilinear fashion as dietary AEE level increased in growing and in finishing pigs ( P  < 0.001). This suggests an influence of EEE on the ATTD of AEE estimates. The linear regression of apparent digested AEE against dietary AEE intake (L1–L5;  P  < 0.001,  R  2  = 0.99 for growing pigs and  P  < 0.001,  R  2  = 0.99 for finishing pigs) estimated greater EEE ( P  < 0.05) and TTTD of AEE ( P  < 0.05) for growing than finishing pigs. Estimated EEE from growing pigs ranged between 18.1 and 20.2 g/kg of DMI, while TTTD of AEE ranged between 96.40% and 100.70%. In finishing pigs, EEE ranged between 21.6 and 23.8 g/kg of DMI and TTTD of AEE ranged between 91.30% and 95.25%. In conclusion, EEE under practical conditions is estimated to be 19.2 g/kg of DMI in growing and 22.7 g/kg of DMI in finishing pigs.

Estimation of endogenous intestinal losses of acid hydrolyzed ether extract in growing and finishing pigs using the linear regression method