An elastin-like recombinamer-based bioactive hydrogel embedded with mesenchymal stromal cells as an injectable scaffold for osteochondral repair | Zendy

Filippo Cipriani | Zendy; Blanca Ariño Palao | Zendy; Israel González de Torre | Zendy; Aurelio Vega Castrillo | Zendy; Héctor J. Hernández | Zendy; Matilde Alonso Rodrigo | Zendy; Ángel José Álvarez Barcia | Zendy; Ana Sánchez | Zendy; Verónica García Diaz | Zendy; Mónica LopezPeña | Zendy; José Carlos RodríguezCabello | Zendy

Open Access

An elastin-like recombinamer-based bioactive hydrogel embedded with mesenchymal stromal cells as an injectable scaffold for osteochondral repair

Author(s) -

Filippo Cipriani,

Blanca Ariño Palao,

Israel González de Torre,

Aurelio Vega Castrillo,

Héctor J. Hernández,

Matilde Alonso Rodrigo,

Ángel José Álvarez Barcia,

Ana Sánchez,

Verónica García Diaz,

Mónica LopezPeña,

José Carlos RodríguezCabello

Publication year - 2019

Publication title -

regenerative biomaterials

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.166

H-Index - 25

ISSN - 2056-3426

DOI - 10.1093/rb/rbz023

Subject(s) - mesenchymal stem cell , regeneration (biology) , elastin , scaffold , biomedical engineering , biomaterial , cartilage , hyaline cartilage , infiltration (hvac) , stromal cell , matrix (chemical analysis) , chemistry , materials science , anatomy , microbiology and biotechnology , pathology , articular cartilage , composite material , medicine , biology , osteoarthritis , alternative medicine , chromatography

The aim of this study was to evaluate injectable, in situ cross-linkable elastin-like recombinamers (ELRs) for osteochondral repair. Both the ELR-based hydrogel alone and the ELR-based hydrogel embedded with rabbit mesenchymal stromal cells (rMSCs) were tested for the regeneration of critical subchondral defects in 10 New Zealand rabbits. Thus, cylindrical osteochondral defects were filled with an aqueous solution of ELRs and the animals sacrificed at 4 months for histological and gross evaluation of features of biomaterial performance, including integration, cellular infiltration, surrounding matrix quality and the new matrix in the defects. Although both approaches helped cartilage regeneration, the results suggest that the specific composition of the rMSC-containing hydrogel permitted adequate bone regeneration, whereas the ELR-based hydrogel alone led to an excellent regeneration of hyaline cartilage. In conclusion, the ELR cross-linker solution can be easily delivered and forms a stable well-integrated hydrogel that supports infiltration and de novo matrix synthesis.

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