Utilizing 3D bioprinted platelet-rich fibrin-based materials to promote the regeneration of oral soft tissue

Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials. Although the injectable platelet-rich fibrin (i-PRF) is a safe, autologous source of high levels of growth factors that is often employed to promote the regeneration of oral soft tissue, its effectiveness is restrained by difficulties in intraoperative shaping together with the burst-like release of growth factors. We herein sought to develop a bioactive bioink composed of i-PRF, alginate, and gelatin capable of promoting the regeneration of the oral soft tissue. This bioink was successfully applied in 3D bioprinting and exhibited its ability to be shaped to individual patient needs. Importantly, we were also able to significantly prolong the duration of multiple growth factors release as compared to that observed for i-PRF. The growth factor bioavailability was further confirmed by the enhanced proliferation and viability of printed gingival fibroblasts. When deployed in vivo in nude mice, this bioink was further confirmed to be biocompatible and to drive enhanced angiogenic activity. Together, these data thus confirm the successful production of an i-PRF-containing bioink, which is suitable for the individualized promotion of the regeneration of oral soft tissue.