Simple application of adipose-derived stem cell-derived extracellular vesicles coating enhances cytocompatibility and osteoinductivity of titanium implant | Zendy

Haiyong Chen | Zendy; Shan Mou | Zendy; Jinfei Hou | Zendy; Huimin Fang | Zendy; Yuyang Zeng | Zendy; Jiaming Sun | Zendy; Zhenxing Wang | Zendy

Open Access

Simple application of adipose-derived stem cell-derived extracellular vesicles coating enhances cytocompatibility and osteoinductivity of titanium implant

Author(s) -

Haiyong Chen,

Shan Mou,

Jinfei Hou,

Huimin Fang,

Yuyang Zeng,

Jiaming Sun,

Zhenxing Wang

Publication year - 2020

Publication title -

regenerative biomaterials

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.166

H-Index - 25

ISSN - 2056-3426

DOI - 10.1093/rb/rbaa038

Subject(s) - mesenchymal stem cell , chemistry , fibronectin , osteoblast , titanium , alkaline phosphatase , adipose tissue , surface modification , stem cell , osseointegration , regenerative medicine , vesicle , extracellular vesicle , implant , biomaterial , osteocalcin , biomedical engineering , microbiology and biotechnology , biophysics , cell , biochemistry , microrna , microvesicles , in vitro , biology , surgery , medicine , membrane , organic chemistry , gene , enzyme

Surface modification using bioactive molecules is frequently performed to improve the biological properties of medical metal biomaterial titanium (Ti) implants. Developmental evidence suggests that mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) served as potent bioactive component. As a subset of MSC-EV, adipose-derived stem cell-derived extracellular vesicles (ADSC-EVs) could be obtained from abundant adipose tissue. Meanwhile, it possesses multiple regenerative properties and might be used to endow biological activities to medical Ti implant. Here, we present a simple ADSC-EV coating strategy based on physisorption of fibronectin. This ADSC-EV functionalized Ti implants (EV-Ti) revealed enhanced osteoblast compatibility and osteoinductive activity. Cell spreading area of EV-Ti group was 1.62- and 1.48-fold larger than that of Ti group after 6 and 12 h of cell seeding, respectively. Moreover, EV-Ti promoted alkaline phosphatase, collagen 1 and osteocalcin gene expression in osteoblast by 1.51-, 1.68- and 1.82-fold compared with pristine Ti, respectively. Thus, the MSC-EVs modification method reported here provide a clinically translatable strategy to promote the bioactivity of Ti implants.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research