Open AccessInteraction of BTB-TAZ protein MdBT2 and DELLA protein MdRGL3a regulates nitrate-mediated plant growth
Author(s) -
YiRan Ren,
Qiang Zhao,
Yuying Yang,
Rui Zhang,
XiaoFei Wang,
Tian-En Zhang,
ChunXiang You,
Heqiang Huo,
YuJin Hao
Publication year - 2021
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1093/plphys/kiab065
Subject(s) - bimolecular fluorescence complementation , arabidopsis , complementation , protein fragment complementation assay , nitrate reductase , proteasome , microbiology and biotechnology , biology , mutant , yeast , plant growth , ubiquitin , biochemistry , heterologous , nitrate , chemistry , botany , enzyme , gene , ecology
Nitrate acts as a vital signal molecule in the modulation of plant growth and development. The phytohormones gibberellin (GA) is also involved in this process. However, the exact molecular mechanism of how nitrate and GA signaling pathway work together in regulating plant growth remains poorly understood. In this study, we found that a nitrate-responsive BTB/TAZ protein MdBT2 participates in regulating nitrate-induced plant growth in apple (Malus × domestica). Yeast two-hybridization, protein pull-down, and bimolecular fluorescence complementation (BiFC) assays showed that MdBT2 interacts with a DELLA protein MdRGL3a, which is required for the ubiquitination and degradation of MdRGL3a proteins via a 26S proteasome-dependent pathway. Furthermore, heterologous expression of MdBT2 partially rescued growth inhibition caused by overexpression of MdRGL3a in Arabidopsis. Taken together, our findings indicate that MdBT2 promotes nitrate-induced plant growth partially through reducing the abundance of the DELLA protein MdRGL3a.
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