Open AccessNon-destructive DNA extraction for small pelagic copepods to perform integrative taxonomy
Author(s) -
Astrid Cornils
Publication year - 2014
Publication title -
journal of plankton research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.87
H-Index - 93
eISSN - 1464-3774
pISSN - 0142-7873
DOI - 10.1093/plankt/fbu105
Subject(s) - pelagic zone , taxonomy (biology) , biology , extraction (chemistry) , copepod , dna barcoding , chromatography , zoology , crustacean , ecology , chemistry
Molecular analyses of small-sized copepods (≤1 mm) generally involve the complete destruction of the specimens. Consequently, incongruences between the molecular and morphological results cannot be investigated since no specimen vouchers remain. The present study provides a modified column-based DNA extraction method to retain the exoskeleton of the specimen and thus, to enable molecular and morphological analysis of the same specimens. The method has been tested on ethanol preserved specimens of nine pelagic copepod genera