Cluster-Level Relationships of Genes Involved in Carbon Metabolism in Synechocystis sp. PCC 6803: Development of a Novel Succinate-Producing Strain | Zendy

Masahiro Takeya | Zendy; Hiroko Iijima | Zendy; Haruna Sukigara | Zendy; Takashi Osanai | Zendy

Open Access

Cluster-Level Relationships of Genes Involved in Carbon Metabolism in Synechocystis sp. PCC 6803: Development of a Novel Succinate-Producing Strain

Author(s) -

Masahiro Takeya,

Hiroko Iijima,

Haruna Sukigara,

Takashi Osanai

Publication year - 2017

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcx162

Subject(s) - mutant , gene , catabolism , strain (injury) , gene expression , regulator , gene cluster , biology , biochemistry , sugar , metabolism , enzyme , chemistry , anatomy

We quantified the transcript levels of 44 genes related to sugar catabolism in strains with altered primary carbon metabolism and discovered a consistent expression pattern among succinate-producing mutants. To identify factors that determine the expression pattern, we calculated Pearson's correlation coefficients, using the transcript data. Correlation analysis revealed positive and negative correlations among genes encoding sugar catabolic enzymes. On the basis of this analysis, we found that the mutant overexpressing both rre37 (encoding an OmpR-type response regulator) and sigE (encoding an RNA polymerase sigma factor) produced increased levels of succinate under dark, anaerobic conditions, with a maximum productivity of 420 mg l-1.

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