Crosstalk between Nitric Oxide and MPK1/2 Mediates Cold Acclimation-induced Chilling Tolerance in Tomato

The participation of nitric oxide (NO) in the responses of plants towards biotic and abiotic stresses is well established. However, the mechanism involved particularly in cold acclimation-induced chilling tolerance remains elusive. Here we show the cold acclimation induced-chilling tolerance was associated with inductions of nitrate reductase (NR)-dependent NO production, S-nitrosylated glutathione reductase (GSNOR) activity and mitogen-activated protein kinases MPK1/2 activation in tomato plants. Silencing of NR resulted in decreased GSNOR activity and MPK1/2 activation, which subsequently compromised cold acclimation-induced chilling tolerance. By contrast, silencing of GSNOR caused decreased NR activity, increased NO accumulation and MPK1/2 activation, and enhanced cold acclimation-induced chilling tolerance. Furthermore, co-silencing of MPK1 and MPK2 attenuated the NR-dependent NO production and cold acclimation-induced tolerance to chilling. Results from present study suggest the importance of MPK1/2 for the induction of NR-dependent NO generation, while the accumulation of nitrosylated glutathione from NO-derived reactive nitrogen species could potentially S-nitrosylate NR. These findings provide new insight into the crosstalk of NO and MPK1/2 in cold acclimation-induced chilling tolerance in tomato plants.