Overexpression of aPhytophthoraCytoplasmic CRN Effector Confers Resistance to Disease, Salinity and Drought inNicotiana benthamiana | Zendy

Nasir Ahmed Rajput | Zendy; Meixiang Zhang | Zendy; Danyu Shen | Zendy; Tingli Liu | Zendy; Qimeng Zhang | Zendy; Yanyan Ru | Zendy; Peng Sun | Zendy; Daolong Dou | Zendy

Open Access

Overexpression of aPhytophthoraCytoplasmic CRN Effector Confers Resistance to Disease, Salinity and Drought inNicotiana benthamiana

Author(s) -

Nasir Ahmed Rajput,

Meixiang Zhang,

Danyu Shen,

Tingli Liu,

Qimeng Zhang,

Yanyan Ru,

Peng Sun,

Daolong Dou

Publication year - 2015

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcv164

Subject(s) - nicotiana benthamiana , oomycete , effector , biology , agroinfiltration , microbiology and biotechnology , biotic stress , abiotic component , phytophthora capsici , plant disease resistance , gene , transgene , phytophthora sojae , phytophthora , botany , genetics , abiotic stress , paleontology

The Crinkler (CRN) effector family is produced by oomycete pathogens and may manipulate host physiological and biochemical events inside host cells. Here, PsCRN161 was identified from Phytophthora sojae based on its broad and strong cell death suppression activities. The effector protein contains two predicted nuclear localization signals and localized to nuclei of plant cells, indicating that it may target plant nuclei to modify host cell physiology and function. The chimeric gene GFP:PsCRN161 driven by the Cauliflower mosaic virus (CaMV) 35S promoter was introduced into Nicotiana benthamiana. The four independent PsCRN161-transgenic lines exhibited increased resistance to two oomycete pathogens (P. parasitica and P. capsici) and showed enhanced tolerance to salinity and drought stresses. Digital gene expression profiling analysis showed that defense-related genes, including ABC transporters, Cyt P450 and receptor-like kinases (RLKs), were significantly up-regulated in PsCRN161-transgenic plants compared with GFP (green fluorescent protein) lines, implying that PsCRN161 expression may protect plants from biotic and abiotic stresses by up-regulation of many defense-related genes. The results reveal previously unknown functions of the oomycete effectors, suggesting that the pathogen effectors could be directly used as functional genes for plant molecular breeding for enhancement of tolerance to biotic and abiotic stresses.

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