Mitochondrial Localization of Ferrochelatase in a Red Alga Cyanidioschyzon merolae | Zendy

Satoru Watanabe | Zendy; Mitsumasa Hanaoka | Zendy; Yusaku Ohba | Zendy; Tomohiro Ono | Zendy; Mio Ohnuma | Zendy; Hirofumi Yoshikawa | Zendy; Shigeru Taketani | Zendy; Kan Tanaka | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Mitochondrial Localization of Ferrochelatase in a Red Alga Cyanidioschyzon merolae

Author(s) -

Satoru Watanabe,

Mitsumasa Hanaoka,

Yusaku Ohba,

Tomohiro Ono,

Mio Ohnuma,

Hirofumi Yoshikawa,

Shigeru Taketani,

Kan Tanaka

Publication year - 2013

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pct077

Subject(s) - ferrochelatase , plastid , subcellular localization , mitochondrion , biology , enzyme , heme , microbiology and biotechnology , chloroplast , biochemistry , cytoplasm , gene

Ferrochelatase (FECH) is an essential enzyme for the final step of heme biosynthesis. In green plants, its activity has been reported in both plastids and mitochondria. However, the precise subcellular localization of FECH remains uncertain. In this study, we analyzed the localization of FECH in the unicellular red alga, Cyanidioschyzon merolae. Immunoblot and enzyme activity analyses of subcellular fractions localized little FECH in the plastid. In addition, immunofluorescence microscopy identified that both intrinsic and hemagglutinin (HA)-tagged FECH are localized in the mitochondrion. We therefore conclude that FECH is localized in the mitochondrion in C. merolae.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research