RNAi-Mediated Knockdown of the XIP-Type Endoxylanase Inhibitor Gene, OsXIP, Has No Effect on Grain Development and Germination in Rice | Zendy

Toko Tokunaga | Zendy; Yoshihiro Miyata | Zendy; Yukichi Fujikawa | Zendy; Muneharu Esaka | Zendy

Open Access

RNAi-Mediated Knockdown of the XIP-Type Endoxylanase Inhibitor Gene, OsXIP, Has No Effect on Grain Development and Germination in Rice

Author(s) -

Toko Tokunaga,

Yoshihiro Miyata,

Yukichi Fujikawa,

Muneharu Esaka

Publication year - 2008

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcn080

Subject(s) - oryza sativa , germination , genetically modified rice , xylanase , gene , biology , transgene , gene knockdown , genetically modified crops , botany , microbiology and biotechnology , biochemistry , enzyme

OsXIP (Oryza sativa xylanase inhibitor protein) is a XIP-type xylanase inhibitor which was identified as a protein encoded by a wound stress-responsive gene in rice. Although the OsXIP gene was specifically expressed in mature grains under basal conditions, recombinant OsXIP had no effect on rice endogenous xylanases, and OsXIP-suppressed transgenic rice plants did not exhibit any change in grain development and germination, suggesting that rice development may be independent of OsXIP. Analysis using an OsXIP-specific antibody revealed that OsXIP is markedly accumulated in apoplast in rice root cells by wounding. These results reinforced the possibility that OsXIP is involved in plant defense mechanisms against phytopathogens.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research