Polyethylene Glycol (PEG)-Mediated Transient Gene Expression in a Red Alga, Cyanidioschyzon merolae 10D | Zendy

Masaaki Ohnuma | Zendy; Takashi Yokoyama | Zendy; Tohru Inouye | Zendy; Yasushi Sekine | Zendy; Kan Tanaka | Zendy

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Polyethylene Glycol (PEG)-Mediated Transient Gene Expression in a Red Alga, Cyanidioschyzon merolae 10D

Author(s) -

Masaaki Ohnuma,

Takashi Yokoyama,

Tohru Inouye,

Yasushi Sekine,

Kan Tanaka

Publication year - 2007

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcm157

Subject(s) - peg ratio , transformation (genetics) , plasmid , polyethylene glycol , gene , intracellular , microbiology and biotechnology , chemistry , flagellum , biology , biophysics , biochemistry , finance , economics

DNA introduction into cells is an essential technique for molecular genetic analysis. Here, we show that DNA is easily introduced into cells of the unicellular red alga Cyanidioschyzon merolae by a polyethylene glycol (PEG)-mediated protocol. In this study, the beta-tubulin gene of C. merolae was cloned on a plasmid and a hemagglutinin (HA) tag then added at the C-terminus. This plasmid was then introduced into C. merolae cells by a PEG-mediated transformation protocol. At 24 h after PEG-mediated transformation, intracellular localization of the tagged protein was detected by anti-HA immunocytochemistry, indicating the utility of this transient expression system for molecular genetic analyses.

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