Contribution of Anaphase B to Chromosome Separation in Higher Plant Cells Estimated by Image Processing | Zendy

Tomomi Hayashi | Zendy; Toshio Sano | Zendy; Natsumaro Kutsuna | Zendy; Fumi Kumagai-Sano | Zendy; Seiichiro Hasezawa | Zendy

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Contribution of Anaphase B to Chromosome Separation in Higher Plant Cells Estimated by Image Processing

Author(s) -

Tomomi Hayashi,

Toshio Sano,

Natsumaro Kutsuna,

Fumi Kumagai-Sano,

Seiichiro Hasezawa

Publication year - 2007

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcm117

Subject(s) - anaphase , microbiology and biotechnology , chromosome , biology , chromosome segregation , elongation , chemistry , genetics , gene , materials science , metallurgy , ultimate tensile strength

Anaphase can be categorized into the two subphases of anaphase A and B, but anaphase B has not been clearly described in higher plant cells. In this study, we time-sequentially followed the dynamics of chromosome segregation and spindle elongation in tobacco BY-2 cells using histone-red fluorescent protein (RFP) and green fluorescent protein (GFP)-tubulin, respectively. Construction of kymographs and determination of the positions of chromosomes and spindle edges by image processing revealed that anaphase B contributed to about 40% of the chromosome separation in distance, which is comparable with that in animal cells. These results suggest that higher plant cells potentially possess the process of anaphase B.

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