Isolation of SIP, a Protein that Interacts with SPB, a Possible Transcriptional Regulatory Factor in Rhodobacter sphaeroides | Zendy

Hiroshi Shimada | Zendy; Kōji Ishida | Zendy; Youhei Machiya | Zendy; K. Takamiya | Zendy

Open Access

Isolation of SIP, a Protein that Interacts with SPB, a Possible Transcriptional Regulatory Factor in Rhodobacter sphaeroides

Author(s) -

Hiroshi Shimada,

Kōji Ishida,

Youhei Machiya,

K. Takamiya

Publication year - 2007

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcm114

Subject(s) - rhodobacter sphaeroides , operon , immunoprecipitation , fusion protein , biology , microbiology and biotechnology , lac operon , plasmid , chemistry , genetics , gene , escherichia coli , bacteria , recombinant dna

SPB is a transcriptional factor in Rhodobacter sphaeroides that represses expression of the puf operon under aerobic or semi-aerobic light conditions. Here, we identified a 17,500 Da protein designated SIP (SPB interaction protein) that interacts with SPB, as determined by binding to an SPB-His(x6) fusion protein-Ni column. The SPB-SIP interaction in vivo was confirmed by an immunoprecipitation assay. The level of transcripts and protein of SIP did not differ for all growth conditions tested, indicating that regulation of the SIP-SPB interaction, if any, is not through modulation of sip or spb expression but rather by modification of the proteins.

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