Disruption of Actin Filaments by Latrunculin B Affects Cell Wall Construction in Picea meyeri Pollen Tube by Disturbing Vesicle Trafficking | Zendy

Tong Chen | Zendy; Nianjun Teng | Zendy; Xiaoqin Wu | Zendy; Yuhua Wang | Zendy; Wei Tang | Zendy; Jozef Šamaj | Zendy; František Baluška | Zendy; Jinxing Lin | Zendy

Open Access

Disruption of Actin Filaments by Latrunculin B Affects Cell Wall Construction in Picea meyeri Pollen Tube by Disturbing Vesicle Trafficking

Author(s) -

Tong Chen,

Nianjun Teng,

Xiaoqin Wu,

Yuhua Wang,

Wei Tang,

Jozef Šamaj,

František Baluška,

Jinxing Lin

Publication year - 2006

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcl036

Subject(s) - pollen tube , microbiology and biotechnology , actin , vesicle , pollen , biology , chemistry , botany , genetics , membrane , pollination

The involvement of actin filaments (AFs) in vesicle trafficking, cell wall construction and tip growth was investigated during pollen tube development of Picea meyeri. Pollen germination and tube elongation were inhibited in a dose-dependent manner by the latrunculin B (LatB) treatment. The fine AFs were broken down into disorganized fragments showing a tendency to aggregate. FM4-64 labeling revealed that the dynamic balance of vesicle trafficking was perturbed due to F-actin disruption and the fountain-like cytoplasmic pattern changed into disorganized Brownian movement. The configuration and/or distribution of cell wall components, such as pectins, callose and cellulose, as well as arabinogalactan proteins changed in obvious ways after the LatB application. Fourier transform infrared (FTIR) analysis further established significant changes in the chemical composition of the wall material. Our results indicate that depolymerization of AFs affects the distribution and configuration of cell wall components in Picea meyeri pollen tube by disturbing vesicle trafficking.

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