The Tomato Photomorphogenetic Mutant, aurea, is Deficient in Phytochromobilin Synthase for Phytochrome Chromophore Biosynthesis | Zendy

Takuya Muramoto | Zendy; Chitose Kami | Zendy; Hideo Kataoka | Zendy; Naoko Iwata | Zendy; Philip J. Linley | Zendy; Keiko Mukougawa | Zendy; Akiho Yokota | Zendy; Takayuki Kohchi | Zendy

Open Access

The Tomato Photomorphogenetic Mutant, aurea, is Deficient in Phytochromobilin Synthase for Phytochrome Chromophore Biosynthesis

Author(s) -

Takuya Muramoto,

Chitose Kami,

Hideo Kataoka,

Naoko Iwata,

Philip J. Linley,

Keiko Mukougawa,

Akiho Yokota,

Takayuki Kohchi

Publication year - 2005

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pci062

Subject(s) - mutant , phytochrome , atp synthase , biosynthesis , biology , gene , biochemistry , arabidopsis , escherichia coli , reductase , microbiology and biotechnology , enzyme , botany , red light

The aurea mutants of tomato have been widely used as phytochrome-deficient mutants for photomorphogenetic and photobiological studies. By expressed sequence tag (EST)-based screening of sequence databases, we found a tomato gene that encodes a protein homologous to Arabidopsis HY2 for phytochromobilin synthase catalyzing the last step of phytochrome chromophore biosynthesis. The tomato protein expressed in Escherichia coli showed phytochromobilin synthase activity. The corresponding loci in all aurea mutants tested have nucleotide substitutions, deletions or DNA rearrangements. These results indicate that aurea is a mutant of phytochromobilin synthase in tomato. We also discuss a phylogenetic analysis of phytochromobilin synthases in the bilin reductase family.

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