Open AccessCharacterization of Full-length Enriched Expressed Sequence Tags of Stress-treated Poplar Leaves
Author(s) -
Tokihiko Nanjo,
Norihiro Futamura,
Mitsuru Nishiguchi,
Tomohiro Igasaki,
Kazuo Shinozaki,
Kenji Shinohara
Publication year - 2004
Publication title -
plant and cell physiology/plant and cell physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.975
H-Index - 152
eISSN - 1471-9053
pISSN - 0032-0781
DOI - 10.1093/pcp/pci009
Subject(s) - expressed sequence tag , cdna library , biology , abscisic acid , complementary dna , botany , salinity , gene , environmental stress , genomics , genome , computational biology , genetics , evolutionary biology , ecology
Poplar, whose genome is the first to be sequenced among woody plants, is a favorable model for plant biologists to enable them to understand molecular processes of growth, development and responses to environmental stimuli in trees. The sequence will allow the development of a strategy for improving environmental stress tolerance in forest trees. In this study, we have generated a full-length enriched cDNA library from leaves of axenically grown poplar (Populus nigra var. italica) subjected to environmental stress treatments by dehydration, high salinity, chilling, heat, abscisic acid (ABA) and H2O2. We sequenced >30,000 expressed sequence tags (ESTs) from the cDNA library and consequently collected approximately 4,500 non-redundant clones. We further analyzed cDNAs encoding an ERF/AP2-domain transcription factor which is specific in plants and plays an important role under stress. Thirteen candidates containing the ERF/AP2 domain were found within our EST resource. Some of them showed stress-responsive gene expression. We report here the first collection of full-length enriched stress-related ESTs of poplar and discuss environmental stress responses of forest trees in the light of comparative genomics.