Sorting Signals in the Cytosolic Tail of Plant p24 Proteins Involved in the Interaction with the COPII Coat | Zendy

Inmaculada Contreras | Zendy; Yaodong Yang | Zendy; David G. Robinson | Zendy; Fernando Aniento | Zendy

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Sorting Signals in the Cytosolic Tail of Plant p24 Proteins Involved in the Interaction with the COPII Coat

Author(s) -

Inmaculada Contreras,

Yaodong Yang,

David G. Robinson,

Fernando Aniento

Publication year - 2004

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pch200

Subject(s) - copii , coat , sorting , protein targeting , cytosol , microbiology and biotechnology , coat protein , chemistry , biology , biophysics , biochemistry , membrane protein , endoplasmic reticulum , membrane , computer science , golgi apparatus , gene , secretory pathway , rna , enzyme , paleontology , programming language

The ability of the cytosolic tail of a plant p24 protein to bind COPI and COPII subunits from plant and animal sources in vitro has been examined. We have found that a dihydrophobic motif in the -7,-8 position (relative to the cytosolic carboxy-terminus), which strongly cooperates with a dilysine motif in the -3,-4 position for COPI binding, is required for COPII binding. In addition, we show that COPI and COPII coat proteins from plant cytosol compete for binding to the sorting motifs in these tails. Only in the absence of the dilysine motif in the -3,-4 position or after COPI depletion could we observe COPII binding to the p24 tail. This competition is not observed when using rat liver cytosol.

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