Functional Analysis of a Rice Putative Voltage-Dependent Ca2+ Channel, OsTPC1, Expressed in Yeast Cells Lacking its Homologous Gene CCH1 | Zendy

Kenji Hashimoto | Zendy; Mikako Saito | Zendy; Hideaki Matsuoka | Zendy; Kazuko Iida | Zendy; Hidetoshi Iida | Zendy

Open Access

Functional Analysis of a Rice Putative Voltage-Dependent Ca2+ Channel, OsTPC1, Expressed in Yeast Cells Lacking its Homologous Gene CCH1

Author(s) -

Kenji Hashimoto,

Mikako Saito,

Hideaki Matsuoka,

Kazuko Iida,

Hidetoshi Iida

Publication year - 2004

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pch053

Subject(s) - heterologous expression , heterologous , yeast , complementary dna , gene , protein subunit , biochemistry , saccharomyces cerevisiae , chemistry , arabidopsis thaliana , verapamil , microbiology and biotechnology , biology , recombinant dna , mutant , calcium , organic chemistry

We isolated a cDNA (OsTPC1) from rice that was homologous to AtTPC1, a putative voltage-dependent Ca(2+) channel (VDCC) gene of Arabidopsis thaliana. The hydropathy profile of its deduced amino acid sequence showed significant structural features of the alpha 1-subunit of animal VDCCs. Functional analysis using a heterologous yeast expression system showed that OsTPC1 facilitates Ca(2+) permeation. The K(m) value for Ca(2+) of OsTPC1, 47.5 micro M, was comparable to that of intrinsic CCH1, a candidate VDCC in yeast. Ca(2+) permeation by OsTPC1 was inhibited by verapamil, a VDCC blocker. These findings indicate for the first time that OsTPC1 is a putative VDCC in rice.

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