Open AccessDistinct Expression and Function of Three Ammonium Transporter Genes (OsAMT1;1 – 1;3) in Rice
Author(s) -
Yutaka Sonoda,
Akira Ikeda,
Satomi Saiki,
Nicolaus von Wirén,
Tomoyuki Yamaya,
Junji Yamaguchi
Publication year - 2003
Publication title -
plant and cell physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.975
H-Index - 152
eISSN - 1471-9053
pISSN - 0032-0781
DOI - 10.1093/pcp/pcg083
Subject(s) - oryza sativa , northern blot , gene , heterologous expression , ammonium , biology , gene expression , mutant , gene family , saccharomyces cerevisiae , arabidopsis , yeast , southern blot , microbiology and biotechnology , botany , genetics , chemistry , organic chemistry , recombinant dna
To study the regulation of ammonium uptake into rice roots, three ammonium transporter genes (OsAMT1;1, 1;2 and 1;3; Oryza sativa ammonium transporter) were isolated and examined. OsAMT1s belong to AMT1 family, containing 11 putative transmembrane-spanning domains. Southern blot analysis and screening of the rice genome database confirmed that with OsAMT1;1-1;3 the complete AMT1 family of rice had been isolated. Heterologous expression of OsAMT1s in the yeast Saccharomyces cerevisiae mutant 31019b showed that all three OsAMT1s exhibit ammonium transport activity. Northern blot analysis showed a distinct expression pattern for the three genes; more constitutive expression in shoots and roots for OsAMT1;1, root-specific and ammonium-inducible expression for OsAMT1;2, and root-specific and nitrogen-derepressible expression for OsAMT1;3. In situ mRNA detection revealed that OsAMT1;2 is expressed in the central cylinder and cell surface of root tips. This gene expression analysis revealed a distinct nitrogen-dependent regulation for AMTs in rice, differing from that in tomato or Arabidopsis:
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