Analysis of Sugars in Squash Xylem Sap | Zendy

Hiroaki Iwai | Zendy; Masako Usui | Zendy; Haruko Hoshino | Zendy; Hiroshi Kamada | Zendy; Toshiro Matsunaga | Zendy; Koichi Kakegawa | Zendy; Tadashi Ishii | Zendy; Shinobu Satoh | Zendy

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Analysis of Sugars in Squash Xylem Sap

Author(s) -

Hiroaki Iwai,

Masako Usui,

Haruko Hoshino,

Hiroshi Kamada,

Toshiro Matsunaga,

Koichi Kakegawa,

Tadashi Ishii,

Shinobu Satoh

Publication year - 2003

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcg075

Subject(s) - xylem , chemistry , arabinogalactan , size exclusion chromatography , chromatography , ammonium , sugar , ion chromatography , ion exchange , column chromatography , ethanol , reagent , shoot , biochemistry , botany , polysaccharide , organic chemistry , ion , biology , enzyme

Xylem sap contains organic and inorganic compounds that might be involved in root-to-shoot communication. To clarify the physiological functions of sugars in xylem sap, we characterized the sugar compounds of the xylem sap. The 80% ethanol-soluble fraction of xylem sap contained mainly myo-inositol and oligosaccharides. The 80% ethanol precipitate was solubilized with cyclohexanediamine tetraacetate and fractionated using anion exchange chromatography. The non-bound fraction from the anion-exchange column reacted with Yariv reagent and was rich in arabinogalactan, indicating the presence of arabinogalactan proteins (AGP). The bound fraction eluted with 50 mM ammonium formate buffer and separated using size exclusion chromatography producing the pectins rhamnogaracturonan (RG)-I and RG-II with apparent molecular masses of 15000 and 11000, respectively. These results indicate that the AGP, RG-I, borate cross-linked RG-II dimer and oligosaccharides produced by root tissues are transported to above-ground organs via xylem sap.

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