Assessment of Utility of Meiosis-Associated Promoters of Lily for Induction of Germinal Ds Transposition in Transgenic Rice | Zendy

Ryouhei Morita | Zendy; Yoko Hattori | Zendy; Shuji Yokoi | Zendy; Hisabumi Takase | Zendy; M. Minami | Zendy; Kazuyuki Hiratsuka | Zendy; Kinya Toriyama | Zendy

Open Access

Assessment of Utility of Meiosis-Associated Promoters of Lily for Induction of Germinal Ds Transposition in Transgenic Rice

Author(s) -

Ryouhei Morita,

Yoko Hattori,

Shuji Yokoi,

Hisabumi Takase,

M. Minami,

Kazuyuki Hiratsuka,

Kinya Toriyama

Publication year - 2003

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcg074

Subject(s) - transposition (logic) , transposable element , somatic cell , promoter , biology , meiosis , genetics , gene , transgene , gene expression , genome , philosophy , linguistics

In order to suppress the somatic excision of the Ds element and increase the independent transposition events of the Ac/Ds transposon tagging system in rice, we employed promoters of two meiosis-specific genes of lily, LIM10 and LIM18. The LIM10 promoter directed GUS expression specifically in anthers, with the LIM18 promoter doing the same in the anthers and somatic tissue. Both promoters induced independent germinal transposition with the frequency of approximately 1%. The LIM10 promoter, lacking induction of somatic transposition, is considered to be useful for improving transposon-tagging efficiencies in rice.

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