An Abundant Periplasmic Protein of the Denitrifying Phototroph Rhodobacter sphaeroides f. sp. denitrificans is PstS, a Component of an ABC Phosphate Transport System | Zendy

Masahiro Matsuzaki | Zendy; Miho Abe | Zendy; Satomi Hara | Zendy; Yoshiko Iwasaki | Zendy; Isamu Yamamoto | Zendy; Toshio Satoh | Zendy

Open Access

An Abundant Periplasmic Protein of the Denitrifying Phototroph Rhodobacter sphaeroides f. sp. denitrificans is PstS, a Component of an ABC Phosphate Transport System

Author(s) -

Masahiro Matsuzaki,

Miho Abe,

Satomi Hara,

Yoshiko Iwasaki,

Isamu Yamamoto,

Toshio Satoh

Publication year - 2003

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcg021

Subject(s) - rhodobacter sphaeroides , periplasmic space , biochemistry , rhodobacter , biology , denitrifying bacteria , paracoccus denitrificans , peptide sequence , rhodospirillaceae , escherichia coli , rhodospirillales , atp binding cassette transporter , gene , microbiology and biotechnology , chemistry , mutant , denitrification , enzyme , transporter , nitrogen , photosynthesis , organic chemistry

To understand a physiological role of an abundant 34-kDa periplasmic protein in the denitrifying phototroph Rhodobacter sphaeroides f. sp. denitrificans grown in a medium containing malate as the carbon source, the gene for the protein was isolated. The deduced amino acid sequence of the protein had a sequence similarity of 66.2% to that of PstS from Sinorhizobium meliloti. The downstream sequence of the Rhodobacter pstS contained five genes similar to pstCAB and phoUB, and its upstream sequence contained a putative regulatory sequence that is analogous to the Pho box involved in phosphate-limitation-induced gene expression in Escherichia coli. Both the amount of the PstS and the pstS promoter-driven expression of lacZ activity increased about two-fold in response to phosphate limitation. This is the first isolation of pst genes encoding proteins of an ABC phosphate transporter system from phototrophic bacteria.

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