Differential Tissue-Specific Response to Sulfate and Methionine of a Soybean Seed Storage Protein Promoter Region in Transgenic Arabidopsis | Zendy

Naoko Ohkama | Zendy; Derek B. Goto | Zendy; Toru Fujiwara | Zendy; Satoshi Naito | Zendy

Open Access

Differential Tissue-Specific Response to Sulfate and Methionine of a Soybean Seed Storage Protein Promoter Region in Transgenic Arabidopsis

Author(s) -

Naoko Ohkama,

Derek B. Goto,

Toru Fujiwara,

Satoshi Naito

Publication year - 2002

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcf149

Subject(s) - cauliflower mosaic virus , green fluorescent protein , transgene , downregulation and upregulation , biology , reporter gene , arabidopsis , microbiology and biotechnology , gene , methionine , arabidopsis thaliana , promoter , storage protein , protein subunit , gene expression , genetically modified crops , mutant , biochemistry , amino acid

Expression of the gene encoding the beta subunit of beta-conglycinin, a major soybean seed storage protein, is upregulated by sulfur deficiency and downregulated by methionine (Met). The tissue-specificity of these regulatory mechanisms was studied using a sulfate-responsive region (beta(SR)) from the beta subunit gene promoter. Transgenic Arabidopsis thaliana lines were generated carrying a green fluorescent protein (GFP) reporter gene under control of the cauliflower mosaic virus 35S RNA promoter with a tandem repeat of the beta(SR) element, referred to as the P35S::beta(SR)x3: GFP transgene. Upregulation of P35S::beta(SR)x3:GFP by sulfur deficiency was strongest in leaf margins, where symptoms of sulfur deficiency first appear. P35S::beta(SR)x3:GFP was also upregulated at 2 d after a medium shift from sulfur-sufficient to sulfur-deficient conditions, suggesting that the chimeric promoter is an efficient indicator of sulfur nutritional status. Analysis of transgene expression in a Met-overaccumulating mto1-1 mutation background revealed that the beta(SR) region carries sufficient information for downregulation of promoter activity by Met in developing seeds, but not in young rosettes. Comparisons with another transgenic line, in which the full-length beta promoter is active in non-seed tissues, also suggested that at least two separate tissue-specific mechanisms exist for the downregulation of the beta promoter by Met.

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