Cloning, Functional Expression, and Mutational Analysis of a cDNA for Lotus japonicus Mitochondrial Phosphate Transporter | Zendy

Kazuki Nakamori | Zendy; Reona Takabatake | Zendy; Yosuke Umehara | Zendy; Hiroshi Kouchi | Zendy; Katsura Izui | Zendy; Shingo Hata | Zendy

Open Access

Cloning, Functional Expression, and Mutational Analysis of a cDNA for Lotus japonicus Mitochondrial Phosphate Transporter

Author(s) -

Kazuki Nakamori,

Reona Takabatake,

Yosuke Umehara,

Hiroshi Kouchi,

Katsura Izui,

Shingo Hata

Publication year - 2002

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcf141

Subject(s) - lotus japonicus , complementary dna , transporter , lotus , biology , biochemistry , cloning (programming) , site directed mutagenesis , microbiology and biotechnology , gene , botany , mutant , computer science , programming language

A full-length cDNA encoding mitochondrial phosphate transporter was isolated from symbiotic nodules of Lotus japonicus, a model legume. The gene appeared to be single-copied and its transcript was abundant in roots, symbiotic root nodules and shoots, in that order. The L. japonicus transporter has a conserved N-ethylmaleimide-reactive Cys residue. Site-directed mutagenesis and phosphate transport assaying in vitro showed that this Cys residue is preferable for the transport activity, although it can be replaced to some extent by Ser, but not by Ala.

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