Salicylic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of Atropa belladonna after Treatment with Exogeneously Added Salicylic Acid | Zendy

Hiroyuki Fukami | Zendy; Tomiko Asakura | Zendy; Hiroshi Hirano | Zendy; Keiko Abe | Zendy; Koichiro Shimomura | Zendy; Takashi Yamakawa | Zendy

Open Access

Salicylic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of Atropa belladonna after Treatment with Exogeneously Added Salicylic Acid

Author(s) -

Hiroyuki Fukami,

Tomiko Asakura,

Hiroshi Hirano,

Keiko Abe,

Koichiro Shimomura,

Takashi Yamakawa

Publication year - 2002

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcf119

Subject(s) - salicylic acid , atropa belladonna , hairy root culture , chemistry , methyltransferase , botany , traditional medicine , biology , biochemistry , medicine , agrobacterium , methylation , gene , transformation (genetics)

In Atropa belladonna hairy roots, exogeneously added salicylic acid (SA) is converted to methyl salicylate (MSA) through the reaction, which might be catalysed by S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase (SAMT). Here we cloned a cDNA for A. belladonna SAMT (AbSAMT1), which consisted of 357 aa residues. It was expressed in E. coli, and the recombinant AbSAMT1 showed SAMT activity. When A. belladonna hairy roots were exposed to a high concentration of SA, AbSAMT1 mRNA begins to be expressed 12 h after the exposure, and steady expression continued over 144 h.

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