Protection of the Oxygen-Evolving Machinery by the Extrinsic Proteins of Photosystem II is Essential for Development of Cellular Thermotolerance in Synechocystis sp. PCC 6803 | Zendy

Aiko Kimura | Zendy; Julian J. EatonRye | Zendy; Eugene Hayato Morita | Zendy; Yoshitaka Nishiyama | Zendy; Hidenori Hayashi | Zendy

Open Access

Protection of the Oxygen-Evolving Machinery by the Extrinsic Proteins of Photosystem II is Essential for Development of Cellular Thermotolerance in Synechocystis sp. PCC 6803

Author(s) -

Aiko Kimura,

Julian J. EatonRye,

Eugene Hayato Morita,

Yoshitaka Nishiyama,

Hidenori Hayashi

Publication year - 2002

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcf110

Subject(s) - photosystem ii , photosystem , synechocystis , photosynthesis , cytochrome , microbiology and biotechnology , oxygen , photosystem i , biology , biochemistry , cyanobacteria , reactive oxygen species , oxygen evolution , chemistry , biophysics , mutagenesis , gene , mutation , mutant , enzyme , genetics , bacteria , organic chemistry , electrode , electrochemistry

The oxygen-evolving machinery of photosystem II in cyanobacteria is associated with three extrinsic proteins: the manganese-stabilizing protein, cytochrome c(550), and PsbU. To elucidate the effect of the presence of these extrinsic proteins on the stabilization of the oxygen-evolving machinery against high-temperature stress, we inactivated the genes for these proteins individually in Synechocystis sp. PCC 6803 by targeted mutagenesis. The thermal stability of the oxygen-evolving machinery decreased in all mutated cells but the extent of the susceptibility to heat inactivation varied between the photosystems lacking the different extrinsic proteins. Cells that lacked either the manganese-stabilizing protein or cytochrome c(550) were unable to enhance the thermal stability of the oxygen-evolving machinery and, moreover, failed to increase cellular thermotolerance when grown at moderately high temperatures. Our findings indicate that the three extrinsic proteins stabilize the oxygen-evolving machinery independently against high-temperature stress and that the thermal stability of the machinery influences cellular thermotolerance.

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