Effects of Light and Low Oxygen Tension on Pigment Biosynthesis in Halobacterium salinarum, Revealed by a Novel Method to Quantify Both Retinal and Carotenoids | Zendy

Wael S. ElSayed | Zendy; Shinichi Takaichi | Zendy; Haruo Saida | Zendy; Masahiro Kamekura | Zendy; Mohamed Abu-Shady | Zendy; Humitake Seki | Zendy; Tomohiko Kuwabara | Zendy

Open Access

Effects of Light and Low Oxygen Tension on Pigment Biosynthesis in Halobacterium salinarum, Revealed by a Novel Method to Quantify Both Retinal and Carotenoids

Author(s) -

Wael S. ElSayed,

Shinichi Takaichi,

Haruo Saida,

Masahiro Kamekura,

Mohamed Abu-Shady,

Humitake Seki,

Tomohiko Kuwabara

Publication year - 2002

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcf044

Subject(s) - biosynthesis , retinal , hydroxylamine , pigment , biochemistry , halobacterium salinarum , chemistry , carotenoid , beta carotene , oxygen tension , oxygen , enzyme , bacteriorhodopsin , organic chemistry , membrane

A novel method for analyzing halobacterial pigments was developed, in which retinal was liberated from halobacterial rhodopsins as retinal oxime by hydroxylamine, ethyl beta-apo-8'-carotenoate was introduced as an internal standard, and the pigments including bacterioruberin and beta-carotene were analyzed by HPLC at the same time. With this method, we revealed that light enhances the biosynthesis of bacterioruberin and the conversion of beta-carotene to retinal, but does not affect beta-carotene biosynthesis in Halobacterium salinarum strain Oyon Moussa-16. Low oxygen tension given in the light brought a slight increase in retinal accumulation, although its biosynthesis from beta-carotene is an oxygenation reaction. This paradox could be explained by the increase in beta-carotene biosynthesis.

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