Experimentally Determined Sequence Requirement of ACGT-Containing Abscisic Acid Response Element | Zendy

Tsukaho Hattori | Zendy; Masako Totsuka | Zendy; Tokunori Hobo | Zendy; Yasuaki Kagaya | Zendy; Yamamoto-Toyoda Akiko | Zendy

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Experimentally Determined Sequence Requirement of ACGT-Containing Abscisic Acid Response Element

Author(s) -

Tsukaho Hattori,

Masako Totsuka,

Tokunori Hobo,

Yasuaki Kagaya,

Yamamoto-Toyoda Akiko

Publication year - 2002

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcf014

Subject(s) - abscisic acid , promoter , chemistry , sequence (biology) , context (archaeology) , heterologous , stereochemistry , consensus sequence , gene , biochemistry , peptide sequence , biology , gene expression , paleontology

The sequence requirement of the ACGT-containing abscisic acid response element (ABRE) was analyzed by systematically substituting the bases surrounding the ACGT-core of motif A, the principal ABRE of the rice gene, OSEM: This was done within the context of a 55-bp promoter fragment that minimally confers ABA-responsiveness to a heterologous promoter. Based on this analysis, the sequence requirement of the ACGT-containing ABRE was determined as ACGTG G/T C, which matched very well with the consensus derived from sequence comparison of ABA-responsive promoters.

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