Isolation and Characterization of Oxygen-Evolving Thylakoid Membranes and Photosystem II Particles from a Glaucocystophyte, Cyanophora paradoxa | Zendy

Mari Shibata | Zendy; Yasuhiro Kashino | Zendy; Kazuhiko Satoh | Zendy; Hiroyuki Koike | Zendy

Open Access

Isolation and Characterization of Oxygen-Evolving Thylakoid Membranes and Photosystem II Particles from a Glaucocystophyte, Cyanophora paradoxa

Author(s) -

Mari Shibata,

Yasuhiro Kashino,

Kazuhiko Satoh,

Hiroyuki Koike

Publication year - 2001

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pce092

Subject(s) - thylakoid , photosystem ii , chemistry , characterization (materials science) , isolation (microbiology) , botany , biophysics , photosynthesis , biology , chloroplast , biochemistry , materials science , nanotechnology , microbiology and biotechnology , gene

Conditions for preparing oxygen-evolving thylakoid membranes and PSII complexes, and those for observing the PSII activity were investigated in a glaucocystophyte, Cyanophora paradoxa. The active thylakoid membranes were isolated either with a medium containing glycerol or with that containing high concentrations of sucrose, phosphate, and citrate. Active PSII particles were solubilized by octyl-beta-D-glucoside from thylakoid membranes and were separated by sucrose density gradient centrifugation. The thylakoid membranes and PSII particles showed an oxygen-evolving activity only in high-ionic-strength media. The extrinsic 33 kDa protein (PsbO) and the cytochrome c(550) (PsbV) were found to be present in the PSII particles as in cyanobacteria or red algae, but no 12 kDa protein (PsbU) was detected. The PsbO protein was classified as a land-plant type by its N-terminal amino acid sequence.

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