Isolation and Purification of the Reaction Center (RC) and the Core (RC-LH1) Complex from Rhodobium marinum: the LH1 Ring of the Detergent-Solubilized Core Complex Contains 32 Bacteriochlorophylls | Zendy

Qian Pu | Zendy; Tatsuo Yagura | Zendy; Yasushi Koyama | Zendy; Richard J. Cogdell | Zendy

Open Access

Isolation and Purification of the Reaction Center (RC) and the Core (RC-LH1) Complex from Rhodobium marinum: the LH1 Ring of the Detergent-Solubilized Core Complex Contains 32 Bacteriochlorophylls

Author(s) -

Qian Pu,

Tatsuo Yagura,

Yasushi Koyama,

Richard J. Cogdell

Publication year - 2000

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/pcd068

Subject(s) - ring (chemistry) , photosynthetic reaction centre , bacteriochlorophyll , chemistry , photosynthesis , stereochemistry , biochemistry , organic chemistry

The reaction center (RC) and the core (RC-LH1) complex were isolated and purified from Rhodobium marinum; together with the LH1 complex [Meckenstock et al. (1992a) FEBS Lett. 311: 128], a complete set of RC, LH1 and RC-LH1 from the same wild-type strain of a purple photosynthetic bacterium can therefore now be made. Comparison of the BChl a/BPhe a ratio (determined by HPLC) between the RC and the RC-LH1 complexes lead us to the determination of the number of BChls in the LH1 ring to be 32.06+/-2.90, indicating that the LH1 ring from Rh. marinum consists of 16 alphabeta subunits.

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