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A rapid and efficient system for Agrobacterium infection-mediated transient gene expression in rice has been developed. Using this system, transient expression of preprophytosulfokine, a precursor of phytosulfokine-a, encoded by OsPSK gene was analyzed. The results suggest that the Agrobacterium infection-mediated transient gene expression system is as efficient in rice Oc cells as in tobacco BY-2 cells and might be useful for rapid analysis not only of foreign gene expression, but also of antisense gene suppression.

plant and cell physiology/plant and cell physiology

A Rapid and Efficient System of Agrobacterium Infection-Mediated Transient Gene Expression in Rice Oc Cells and Its Application for Analysis of the Expression and Antisense Suppression of Preprophytosulfokine, a Precursor of Phytosulfokine- , Encoded by OsPSK Gene