Open AccessA Rapid and Efficient System of Agrobacterium Infection-Mediated Transient Gene Expression in Rice Oc Cells and Its Application for Analysis of the Expression and Antisense Suppression of Preprophytosulfokine, a Precursor of Phytosulfokine- , Encoded by OsPSK Gene
Author(s) -
Heping Yang,
Akiko Morita,
Yoshikatsu Matsubayashi,
K. Nakamura,
Youji Sakagami
Publication year - 2000
Publication title -
plant and cell physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.975
H-Index - 152
eISSN - 1471-9053
pISSN - 0032-0781
DOI - 10.1093/pcp/41.6.811
Subject(s) - agrobacterium , gene , gene expression , biology , transient (computer programming) , microbiology and biotechnology , genetics , transformation (genetics) , computer science , operating system
A rapid and efficient system for Agrobacterium infection-mediated transient gene expression in rice has been developed. Using this system, transient expression of preprophytosulfokine, a precursor of phytosulfokine-a, encoded by OsPSK gene was analyzed. The results suggest that the Agrobacterium infection-mediated transient gene expression system is as efficient in rice Oc cells as in tobacco BY-2 cells and might be useful for rapid analysis not only of foreign gene expression, but also of antisense gene suppression.
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