Open AccessMolecular Cloning and Characterization of a cDNA Encoding Proline Transporter in Rice
Author(s) -
Yumiko Igarashi,
Yoshu Yoshiba,
Tomoko Takeshita,
Shinichiro M. Nomura,
Jun Otomo,
Kazuko YamaguchiShinozaki,
Kazuo Shinozaki
Publication year - 2000
Publication title -
plant and cell physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.975
H-Index - 152
eISSN - 1471-9053
pISSN - 0032-0781
DOI - 10.1093/pcp/41.6.750
Subject(s) - complementary dna , cdna library , northern blot , biology , microbiology and biotechnology , gene , homology (biology) , oryza sativa , molecular cloning , proline , southern blot , lycopersicon , amino acid , biochemistry , peptide sequence , western blot , gene expression , botany
A cDNA encoding a proline (Pro) transporter (ProT) was isolated and characterized from a cDNA library prepared from 14-d-old seedlings of Oryza sativa cv. Akibare. The deduced amino acid sequence of the rice ProT protein (OsProT) had 68.8% homology to the ProT protein 1 from Arabidopsis thaliana and 59.6% homology to that from Lycopersicon esculentum. Northern blot analysis revealed that the gene for OsProT (OsProT) was expressed in all organs examined, comparatively strongly in leaf sheath and stem. Salt treatment did not induce expression of OsProT but strongly induced expression of the gene for delta1-pyrroline-5-carboxylate synthetase (P5CS), a key enzyme in Pro biosynthesis. Southern blot analysis revealed that OsProT has a gene family. OsProT specifically transported L-Pro in a transport assay using Xenopus laevis oocytes.
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