Isolation and Characterization of Arsenate-Sensitive and Resistant Mutants of Chlamydomonas reinhardtii | Zendy

Shoko Fujiwara | Zendy; Isao Kobayashi | Zendy; S. Hoshino | Zendy; Toshikazu Kaise | Zendy; Kousuke Shimogawara | Zendy; Hideaki Usuda | Zendy; M Tsuzuki | Zendy

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Isolation and Characterization of Arsenate-Sensitive and Resistant Mutants of Chlamydomonas reinhardtii

Author(s) -

Shoko Fujiwara,

Isao Kobayashi,

S. Hoshino,

Toshikazu Kaise,

Kousuke Shimogawara,

Hideaki Usuda,

M Tsuzuki

Publication year - 2000

Publication title -

plant and cell physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.975

H-Index - 152

eISSN - 1471-9053

pISSN - 0032-0781

DOI - 10.1093/pcp/41.1.77

Subject(s) - arsenate , arsenic , chlamydomonas reinhardtii , mutant , chemistry , insertional mutagenesis , biochemistry , intracellular , mutagenesis , arsenobetaine , sodium arsenate , biology , gene , organic chemistry

Arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii were obtained by screening mutants generated by random insertional mutagenesis for growth in the presence of various concentrations of arsenate. The intracellular concentrations of arsenic in the mutants kept in the arsenate-containing medium were determined with an atomic absorption spectrophotometer. The intracellular levels of arsenic in the arsenate-resistant mutants were all lower than that of the parent strain CC425. Some of the arsenate-sensitive mutants, AS1 and AS3, showed obviously higher levels of arsenic than that of CC425, while other sensitive mutant, AS2, did not accumulate arsenic so much. Analysis of the chemical species of arsenic suggested that inorganic arsenic was converted to dimethylarsinic acid (DMAA) in CC425. However, DMAA was hardly detected in AS2. The mechanisms of the resistance to arsenate are discussed on its uptake and detoxification.

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