Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides | Zendy

Ameneh Khatami | Zendy; Tara M. Randis | Zendy; Anna B. Chamby | Zendy; Thomas A. Hooven | Zendy; Margaret Gegick | Zendy; Evan Suzman | Zendy; Brady A’Hearn-Thomas | Zendy; Andrew P. Steenhoff | Zendy; Adam J. Ratner | Zendy

Open Access

Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides

Author(s) -

Ameneh Khatami,

Tara M. Randis,

Anna B. Chamby,

Thomas A. Hooven,

Margaret Gegick,

Evan Suzman,

Brady A’Hearn-Thomas,

Andrew P. Steenhoff,

Adam J. Ratner

Publication year - 2018

Publication title -

open forum infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.546

H-Index - 35

ISSN - 2328-8957

DOI - 10.1093/ofid/ofy164

Subject(s) - streptococcus agalactiae , streptococcus , polymerase chain reaction , oligonucleotide , medicine , real time polymerase chain reaction , group b , gene , group a , genome , computational biology , pathogen , whole genome sequencing , consensus sequence , microbiology and biotechnology , genetics , biology , base sequence , immunology , bacteria

Group B (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including . We demonstrate suboptimal sensitivity of the existing PCR strategy and validate an improved method based on consensus sequences from >100 GBS genomes.

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