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Group B  (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including . We demonstrate suboptimal sensitivity of the existing  PCR strategy and validate an improved method based on consensus sequences from >100 GBS genomes.

Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides