Metagenomic Sequencing of an Echovirus 30 Genome From Cerebrospinal Fluid of a Patient With Aseptic Meningitis and Orchitis | Zendy

Anne Piantadosi | Zendy; Shibani S. Mukerji | Zendy; Pooja Chitneni | Zendy; Tracey Cho | Zendy; Lisa A. Cosimi | Zendy; Deborah T. Hung | Zendy; Marcia B. Goldberg | Zendy; Pardis C. Sabeti | Zendy; Daniel R. Kuritzkes | Zendy; Yonatan H. Grad | Zendy

Open Access

Metagenomic Sequencing of an Echovirus 30 Genome From Cerebrospinal Fluid of a Patient With Aseptic Meningitis and Orchitis

Author(s) -

Anne Piantadosi,

Shibani S. Mukerji,

Pooja Chitneni,

Tracey Cho,

Lisa A. Cosimi,

Deborah T. Hung,

Marcia B. Goldberg,

Pardis C. Sabeti,

Daniel R. Kuritzkes,

Yonatan H. Grad

Publication year - 2017

Publication title -

open forum infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.546

H-Index - 35

ISSN - 2328-8957

DOI - 10.1093/ofid/ofx138

Subject(s) - aseptic meningitis , medicine , orchitis , cerebrospinal fluid , meningitis , echovirus , aseptic processing , metagenomics , virology , asepsis , microbiology and biotechnology , pathology , enterovirus , virus , pediatrics , biology , genetics , surgery , gene

Enteroviruses cause a wide spectrum of clinical disease. In this study, we describe the case of a young man with orchitis and aseptic meningitis who was diagnosed with enterovirus infection. Using unbiased "metagenomic" massively parallel sequencing, we assembled a near-complete viral genome, the first use of this method for full-genome viral sequencing from cerebrospinal fluid. We found that the genome belonged to the subgroup echovirus 30, which is a common cause of aseptic meningitis but has not been previously reported to cause orchitis.

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