T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT? | Zendy

Chris D. Lauruschkat | Zendy; Lukas Page | Zendy; Sonja Etter | Zendy; Philipp Weis | Zendy; Florian Gamon | Zendy; Sabrina Kraus | Zendy; Hermann Einsele | Zendy; Sebastian Wurster | Zendy; Juergen Loeffler | Zendy

Open Access

T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT?

Author(s) -

Chris D. Lauruschkat,

Lukas Page,

Sonja Etter,

Philipp Weis,

Florian Gamon,

Sabrina Kraus,

Hermann Einsele,

Sebastian Wurster,

Juergen Loeffler

Publication year - 2020

Publication title -

open forum infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.546

H-Index - 35

ISSN - 2328-8957

DOI - 10.1093/ofid/ofaa547

Subject(s) - elispot , medicine , immunology , serology , concordance , immune system , stem cell , cytomegalovirus , lymphocytopenia , immunoassay , t cell , human immunodeficiency virus (hiv) , antibody , lymphocyte , herpesviridae , biology , viral disease , genetics

We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus-reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood–based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology.

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